Cryoablation-induced neutrophil Ca²⁺ elevation and NET formation exacerbate immune escape in colorectal cancer liver metastasis.

ABSTRACT:

Background

Liver metastasis poses a significant barrier to effective immunotherapy in patients with colorectal cancer. Cryoablation has emerged as a vital supplementary therapeutic approach for these patients. However, its impact on the tumor microenvironment following the ablation of liver metastases remains unclear.

Methods

We acquired multi-omics time-series data at 1 day, 5 days, and 14 days post-cryoablation, based on tumor and peripheral blood samples from clinical patients, cell co-culture models, and a liver metastases mouse model built on the MC38 cell line in C57BL/6 J mice. This dataset included single-cell transcriptomic sequencing, bulk tissue transcriptomic sequencing, 4D-Label-Free proteomics, flow cytometry data, western blot data, and histological immunofluo

SUBMITTER: Tan H 

PROVIDER: S-EPMC11626751 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature