STIM1 functions as a proton sensor to coordinate cytosolic pH with store-operated calcium entry.

ABSTRACT: The meticulous regulation of intracellular pH (pH_i) is crucial for maintaining cellular function and homeostasis, impacting physiological processes such as heart rhythm, cell migration, proliferation, and differentiation. Dysregulation of pH_i is implicated in various pathologies such as arrhythmias, cancer, and neurodegenerative diseases. Here, we explore the role of STIM1, an ER calcium (Ca²⁺) sensor mediating Store Operated Ca²⁺ Entry (SOCE), in sensing pH_i changes. Our study reveals that STIM1 functions as a sensor for pH_i changes, independent of its Ca²⁺-binding state. Through comprehensive experimental approaches including confocal microscopy, FRET-based sensors, and mutagenesis, we demonstrate that changes i

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11626807 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature