Reconstruction and analyses of genome-scale <i>halomonas</i> metabolic network yield a highly efficient PHA production.

ABSTRACT: In pursuit of reliable and efficient industrial microbes, this study integrates cutting-edge systems biology tools with Halomonas bluephagenesis TD01, a robust halophilic bacterium. We generated the complete and annotated circular genome sequence for this model organism, constructed and meticulously curated a genome-scale metabolic network, achieving striking 86.32% agreement with Biolog Phenotype Microarray data and visualize the network via an interactive Electron/Thrift server architecture. We then analyzed the genome-scale network using vertex sampling analysis (VSA) and found that productions of biomass, polyhydroxyalkanoates (PHA), citrate, acetate, and pyruvate are mutually competing. Recognizing the dynamic nature of H. bluephagenesis TD01, we further developed and im

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11626823 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature