CD4⁺ T cells exhibit distinct transcriptional phenotypes in the lymph nodes and blood following mRNA vaccination in humans.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and mRNA vaccination induce robust CD4⁺ T cell responses. Using single-cell transcriptomics, here, we evaluated CD4⁺ T cells specific for the SARS-CoV-2 spike protein in the blood and draining lymph nodes (dLNs) of individuals 3 months and 6 months after vaccination with the BNT162b2 mRNA vaccine. We analyzed 1,277 spike-specific CD4⁺ T cells, including 238 defined using Trex, a deep learning-based reverse epitope mapping method to predict antigen specificity. Human dLN spike-specific CD4⁺ follicular helper T (T_FH) cells exhibited heterogeneous phenotypes, including germinal center CD4⁺ T_FH cells and CD4⁺IL-10⁺ T_FH

SUBMITTER: Borcherding N 

PROVIDER: S-EPMC11627549 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature