Endoplasmic reticulum stress triggers unfolded protein response as an antiviral strategy of teleost erythrocytes.

ABSTRACT:

Introduction

Fish nucleated red blood cells (RBCs), also known as erythrocytes, play a crucial role in maintaining immune system balance by modulating protein expression in response to various stimuli, including viral attack. This study explores the intriguing behavior of rainbow trout RBCs when faced with the viral hemorrhagic septicemia virus (VHSV), focusing on the endoplasmic reticulum (ER) stress and the unfolded protein response (UPR).

Methods

Rainbow trout RBCs were Ficoll-purified and exposed to ultraviolet (UV)-inactivated VHSV or live VHSV at different multiplicities of infection (MOIs). Using cryo-soft X-ray tomography (cryo-SXT), we uncovered structural and cellular modifications in RBCs exposed to UV-inactivated VHSV. Moreover, RBCs were treated with 4-phenylbut

SUBMITTER: Salvador-Mira M 

PROVIDER: S-EPMC11628393 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature