Multifocal lipid membrane characterization by combination of DAS-deconvolution and anisotropy.

ABSTRACT: Three analog solvatochromic probes, Laurdan, Prodan, and Acdan, are extensively used in the study of biological sciences. Their locations in lipid membranes vary greatly in depth, and their fluorescence responds to their surrounding environment based on their corresponding locations in the membrane. Utilizing the fluorescence lifetimes (τ) and emission peak positions (λ) acquired from the time-resolved emission spectrum, one can effectively determine the local lipid environment using the analytical approach, referred to as τ and λ plots. Herein, a τ and λ plot was created using the aforementioned probes to expand the analytical field according to their location. Furthermore, the solvent modeling method in the τ and λ plot was upgraded to artificially emulate the complex environment in lipi

SUBMITTER: Ito N 

PROVIDER: S-EPMC11628859 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature