CAR-NK cells derived from cord blood originate mainly from CD56^-CD7⁺CD34^-HLA-DR^-Lin^- NK progenitor cells.

ABSTRACT: Cord blood (CB)-derived chimeric antigen receptor (CAR)-natural killer (NK) cells targeting CD19 have been shown to be effective against B cell malignancies. While human CD56⁺ NK cells can be expanded in vitro, NK cells can also be differentiated from hematopoietic progenitor cells. It is still unclear whether CAR-NK cells originate from mature NK cells or NK progenitor cells in CB. Here, we determined that CAR-NK cells were predominantly derived from CD56^- NK progenitor cells. We first found that substantial numbers of CD19 CAR-NK cells were produced from CD56^- CB mononuclear cells after in vitro culture for 2 weeks. Single-cell RNA sequencing analysis of CD56^-CD3^-CD14^-CD19^- CB mononuclear cells rev

SUBMITTER: Wibowo T 

PROVIDER: S-EPMC11629225 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature