MST1 interactomes profiling across cell death in esophageal squamous cell carcinoma.

ABSTRACT:

Objectives

Resistance to apoptosis in esophageal squamous cell carcinoma (ESCC) constitutes a significant impediment to treatment efficacy. Exploring alternative cell death pathways and their regulatory factors beyond apoptosis is crucial for overcoming drug resistance and enhancing therapeutic outcomes in ESCC.

Methods

Mammalian Ste 20-like kinase 1 (MST1) is implicated in regulating various cell deaths, including apoptosis, autophagy, and pyroptosis. Employing enhanced ascorbate peroxidase 2 (APEX2) proximity labeling coupled with immunoprecipitation-mass spectrometry (IP-MS), we elucidated the interactomes of MST1 across these three cell death paradigms.

Results

Proteomic profiling unveiled the functional roles and subcellular localization of MST1 and its interacti

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11629308 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature