Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer.

ABSTRACT: Esophageal squamous cell carcinoma (ESCC) is a common malignancy, characterized by a multistep pathogenic process regulated spatiotemporally within the esophageal epithelial microenvironment, including vessel normalization and immune infiltration. However, empirical evidence elucidating esophageal vascular remodeling and immune infiltration during ESCC tumorigenesis in situ is lacking. In this study, utilizing a mouse model recapitulating progressive human ESCC stages, we established a tissue clearing workflow for three-dimensional visualization and analysis of esophageal vessels and T cell distribution. Through this workflow, we delineated the spatial dynamics of vascular remodeling, CD3⁺ T cells, and characteristic T cell aggregates employing high-resolution light-sheet

SUBMITTER: Bi S 

PROVIDER: S-EPMC11629339 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature