Elevation of Granulocyte Colony Stimulating Factor in Human AMD Donor RPE-Choroid.

ABSTRACT:

Purpose

Choroidal inflammation, complement deposition, and accumulation of C-reactive protein (CRP) are involved in age-related macular degeneration (AMD) pathology. The pro-inflammatory signals that regulate immune cell recruitment in the choroid of patients with AMD remain to be determined. We performed cytokine profiling of human AMD and age-matched control donor tissue to identify inflammatory molecules upregulated in AMD tissue.

Methods

Protein was isolated from 25 AMD and 21 control donor RPE/choroid macular punches. Total protein was quantified, and 50 µg assayed for expression of 40 cytokines using an inflammation array. We validated the elevated expression of granulocyte colony stimulating factor (G-CSF) protein by ELISA in a second cohort of 22 control and 26 AMD d

SUBMITTER: Mulfaul K 

PROVIDER: S-EPMC11629913 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature