Novel mechanisms of strigolactone-induced DWARF14 degradation in Arabidopsis thaliana.

ABSTRACT: In angiosperms, the strigolactone receptor is the α/β hydrolase DWARF14 (D14) that, upon strigolactone binding, undergoes conformational changes, triggers strigolactone-dependent responses, and hydrolyses strigolactones. Strigolactone signalling involves the formation of a complex between strigolactone-bound D14, the E3-ubiquitin ligase SCFMAX2, and the transcriptional corepressors SMXL6/7/8, which become ubiquitinated and degraded by the proteasome. Strigolactone also destabilizes the D14 receptor. The current model proposes that D14 degradation occurs after ubiquitination of the SMXLs via SCFMAX2 and proteasomal degradation. Using fluorescence and luminescence assays on transgenic lines expressing D14 fused to GREEN FLUORESCENT PROTEIN or LUCIFERASE, we showed that strigolactone-induced

SUBMITTER: Sanchez Martin-Fontecha E 

PROVIDER: S-EPMC11630080 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature