Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii.

ABSTRACT: The yeast Komagataella phaffii has become a popular host strain among biotechnology start-up companies for producing recombinant proteins for food and adult nutrition applications. Komagataella phaffii is a host of choice due to its long history of safe use, open access to protocols and strains, a secretome free of host proteins and proteases, and contract manufacturing organizations with deep knowledge in bioprocess scale-up. However, a recent publication highlighted the abundance of an unknown polysaccharide that accumulates in the supernatant during fermentation. This poses a significant challenge in using K. phaffii as a production host. This polysaccharide leads to difficulties in achieving high purity products and requires specialized and costly downstream processing steps for remova

SUBMITTER: Fischer A 

PROVIDER: S-EPMC11630240 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature