Tagitinin A regulates an F-box gene, CPR30, to resist tomato spotted wilt orthotospovirus (TSWV) infection in Nicotiana benthamiana.

ABSTRACT: Tomato spotted wilt orthotospovirus (TSWV) is one of the most destructive pathogens and causes serious losses in agriculture worldwide. Biogenic pesticides application may be an effective approach for defending against TSWV. Tagitinin A (Tag A) extracted from Tithonia diversifolia (Hemsl.) A. Gray has a high protective effect against TSWV infection. Tag A can induce jasmonic acid to suppress gene expression in TSWV. In this study, the F-box protein (CPR30) was mediated by Tag A, the expression of the CPR30 gene in Tag A-treated leaves was significantly higher (2 times) than that of the negative control. Furthermore, the replication of TSWV-NSm/NSs genes and the expression of TSWV-NSm/NSs proteins significantly increased after silencing the CPR30 gene in protective assays; CPR30 overexpress

SUBMITTER: Li J 

PROVIDER: S-EPMC11630581 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature