Determination of metal ion transport rate of human ZIP4 using stable zinc isotopes.

ABSTRACT: The essential microelement zinc is absorbed in the small intestine mainly by the zinc transporter ZIP4, a representative member of the Zrt/Irt-like protein (ZIP) family. ZIP4 is reportedly upregulated in many cancers, making it a promising oncology drug target. To date, there have been no reports on the turnover number of ZIP4, which is a crucial missing piece of information needed to better understand the transport mechanism. In this work, we used a nonradioactive zinc isotope, ⁷⁰Zn, and inductively coupled plasma mass spectrometry to study human ZIP4 (hZIP4) expressed in Human embryonic kidney 293 cells. Our data showed that ⁷⁰Zn can replace the radioactive ⁶⁵Zn as a tracer in kinetic evaluation of hZIP4 activity. This approach, combined with the quantifi

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11630640 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature