Impact of fixation duration on messenger RNA detectability in human formalin-fixed paraffin-embedded brain tissue.

ABSTRACT: Technologies to study mRNA in post-mortem human brain samples have greatly advanced our understanding of brain pathologies. With ongoing improvements, particularly in formalin-fixed paraffin-embedded tissue, these technologies will continue to enhance our knowledge in the future. Despite various considerations for tissue and mRNA quality, such as pre-mortem health status and RNA integrity, the impact of the tissue fixation time has not been addressed in a systemic fashion yet. In this study, we employed RNAscope to assess mRNA detectability in human post-mortem brain tissue in relation to fixation time. Our results reveal a dynamic change in mRNA detection across varying fixation durations, accompanied by an increase in signal derived from the negative probe and autofluorescence background

SUBMITTER: Hurler CA 

PROVIDER: S-EPMC11630792 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature