Paracrine rescue of MYR1-deficient <i>Toxoplasma gondii</i> mutants reveals limitations of pooled <i>in vivo</i> CRISPR screens.

ABSTRACT: Toxoplasma gondii is an intracellular parasite that subverts host cell functions via secreted virulence factors. Up to 70% of parasite-controlled changes in the host transcriptome rely on the MYR1 protein, which is required for the translocation of secreted proteins into the host cell. Mice infected with MYR1 knock-out (KO) strains survive infection, supporting a paramount function of MYR1-dependent secreted proteins in Toxoplasma virulence and proliferation. However, we have previously shown that MYR1 mutants have no growth defect in pooled in vivo CRISPR-Cas9 screens in mice, suggesting that the presence of parasites that are wild-type at the myr1 locus in pooled screens can rescue the phenotype. Here, we demonstrate that MYR1 is not required for the survival

SUBMITTER: Torelli F 

PROVIDER: S-EPMC11630813 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature