MiR-137 regulates autophagy and apoptosis in duodenal ulcer by targeting BNIP3L.

ABSTRACT:

Background

Duodenal ulcer (DU) represents a clinical manifestation and disease state that occurs when the mucosal surface of the duodenum is damaged. The processes of autophagy and apoptosis have been linked to the development of DU, yet the precise roles they play remain unclear. This study aimed to investigate the expression and mechanism of action of microRNAs (miRNA)-137 (miR-137) in DU.

Methods

Dysregulated miRNAs and targeted genes were identified from the Gene Expression Omnibus database, and the immune cell infiltration levels were analyzed using CIBERSORT. To confirm the targeting of the miRNAs, we conducted dual luciferase reporter assays in vitro. The detection of cell apoptosis was conducted using flow cytometry. Moreover, quantitative reverse transcription polym

SUBMITTER: Pan Z 

PROVIDER: S-EPMC11630971 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature