Tracking the uptake of labelled host-derived extracellular vesicles by the human fungal pathogen <i>Aspergillus fumigatus</i>.

ABSTRACT: Extracellular vesicles (EVs) have gained attention as facilitators of intercellular as well as interkingdom communication during host-microbe interactions. Recently we showed that upon infection, host polymorphonuclear leukocytes produce antifungal EVs targeting the clinically important fungal pathogen Aspergillus fumigatus; however, the small size of EVs (<1 µm) complicates their functional analysis. Here, we employed a more tractable, reporter-based system to label host alveolar epithelial cell-derived EVs and enable their visualization during in vitro A. fumigatus interaction. Fusion of EV marker proteins (CD63, CD9, and CD81) with a Nanoluciferase (NLuc) and a green fluorescent protein (GFP) facilitated their relative quantification by luminescence and visualization by a

SUBMITTER: Visser C 

PROVIDER: S-EPMC11631206 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature