A rapid and inexpensive 96-well DNA-extraction method from blood using silicon dioxide powder (Glassmilk).

ABSTRACT: We present a rapid high-throughput DNA extraction method for use with EDTA-anticoagulated blood using silicon dioxide (SiO₂) powder in a guanidine-HCl solution, hereinafter referred to as "Glassmilk." The method utilizes a 96-well deep-well plate, enabling DNA extraction from 96 samples in under 3 h. The method integrates cell lysis, washing, elution, and DNA storage within the same well, eliminating the need for DNA transfer. The Glassmilk extraction method is cost-effective and fast, and it avoids expensive or toxic reagents by using only basic lab equipment. The method yielded approximately 40 μg of high-quality DNA from 200 μl of blood. The DNA yield of the Glassmilk method was about 50% higher, and the purity of the DNA was comparable to those obtained using two commercial

SUBMITTER: Vasquez Bonilla MM 

PROVIDER: S-EPMC11631390 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature