The distribution of beneficial mutational effects between two sister yeast species poorly explains natural outcomes of vineyard adaptation.

ABSTRACT: Domesticated strains of Saccharomyces cerevisiae have adapted to resist copper and sulfite, two chemical stressors commonly used in winemaking. S. paradoxus has not adapted to these chemicals despite being consistently present in sympatry with S. cerevisiae in vineyards. This contrast could be driven by a number of factors including niche differences or differential access to resistance mutations between species. In this study, we used a comparative mutagenesis approach to test whether S. paradoxus is mutationally constrained with respect to acquiring greater copper and sulfite resistance. For both species, we assayed the rate, effect size, and pleiotropic costs of resistance mutations and sequenced a subset of 150 mutants. We found that the distributions of mutational effects displayed by

SUBMITTER: Longan ER 

PROVIDER: S-EPMC11631397 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature