Negative binomial mixture model for identification of noise in antibody-antigen specificity predictions from single-cell data.

ABSTRACT:

Motivation

LIBRA-seq (linking B cell receptor to antigen specificity by sequencing) provides a powerful tool for interrogating the antigen-specific B cell compartment and identifying antibodies against antigen targets of interest. Identification of noise in single-cell B cell receptor sequencing data, such as LIBRA-seq, is critical for improving antigen binding predictions for downstream applications including antibody discovery and machine learning technologies.

Results

In this study, we present a method for denoising LIBRA-seq data by clustering antigen counts into signal and noise components with a negative binomial mixture model. This approach leverages single-cell sequencing reads from a large, multi-donor dataset described in a recent LIBRA-seq study to develop a data-

SUBMITTER: Wasdin PT 

PROVIDER: S-EPMC11631427 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature