Development of a new breath collection method for analyzing volatile organic compounds from intubated mouse models.

ABSTRACT: A new pre-clinical method for capturing breath samples from intubated mice is presented. This method significantly reduces background levels, allowing more accurate measurements of VOCs originating from the breath ("on-breath") as opposed to background contamination. The method was developed by integrating industry-standard volatile-capturing sorbent tubes with respiratory mechanics measurement equipment (flexiVent^®), resulting in a mouse breath sample that can be transported and analyzed by TD-GC-MS and other central lab technologies. Using the methodology, the discrimination between on-breath VOCs from background compounds provides a cleaner dataset, which can accelerate the validation of VOCs identified from mouse models and their translation to clinical trials. Three metrics

SUBMITTER: Taylor A 

PROVIDER: S-EPMC11631442 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature