Ranking antibody binding epitopes and proteins across samples from whole proteome tiled linear peptides.

ABSTRACT:

Introduction

Ultradense peptide binding arrays that can probe millions of linear peptides comprising the entire proteomes of human or mouse, or hundreds of thousands of microbes, are powerful tools for studying the antibody repertoire in serum samples to understand adaptive immune responses.

Motivation

There are few tools for exploring high-dimensional, significant and reproducible antibody targets for ultradense peptide binding arrays at the linear peptide, epitope (grouping of adjacent peptides), and protein level across multiple samples/subjects (i.e. epitope spread or immunogenic regions of proteins) for understanding the heterogeneity of immune responses.

Results

We developed Hierarchical antibody binding Epitopes and pROteins from liNear peptides (HERON), an R p

SUBMITTER: McIlwain SJ 

PROVIDER: S-EPMC11631460 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature