Use of qPCR to monitor 2,4-dinitroanisole degrading bacteria in water and soil slurry cultures.

ABSTRACT: Prediction and process monitoring during natural attenuation, bioremediation, and biotreatment require effective strategies for detection and enumeration of the responsible bacteria. The use of 2,4-dinitroanisole (DNAN) as a component of insensitive munitions leads to environmental contamination of firing ranges and manufacturing waste streams. Nocardioides sp. strain JS1661 degrades DNAN under aerobic conditions via a pathway involving an unusual DNAN demethylase. We used the deeply branched sequences of DNAN degradation functional genes as a target for development of a molecular method for detection of the bacteria. A qPCR assay was designed for the junction between dnhA and dnhB, the adjacent genes encoding DNAN demethylase. The assay allowed reproducible enumeration of JS1661 during gr

SUBMITTER: Waidner LA 

PROVIDER: S-EPMC11631463 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature