Efficient prime editing in two-cell mouse embryos using PEmbryo.

ABSTRACT: Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice.

SUBMITTER: Kim-Yip RP 

PROVIDER: S-EPMC11631759 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature