Aging and cell expansion enhance microRNA diversity in small extracellular vesicles produced from human adipose-derived stem cells.

ABSTRACT: Adipose-derived stem cells (ASCs) and their small extracellular vesicles (sEVs) hold significant potential for regenerative medicine due to their tissue repair capabilities. The microRNA (miRNA) content in sEVs varies depending on ASC status; however, the effects of aging and cell passage on miRNA profiles remain unclear. In this study, we examined the effects of donor age and cell expansion on ASC characteristics and transcriptome using ASCs obtained from three young and three old donors. Cell expansion significantly impaired stem cell properties, notably reducing proliferation and differentiation capacities. In contrast, donor age had minimal effects on ASCs. RNA sequencing (RNA-seq) revealed differences in gene expression related to stemness, phagocytosis, and metabolic processes influe

SUBMITTER: Tsubaki T 

PROVIDER: S-EPMC11631832 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature