Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in Methylococcus capsulatus.

ABSTRACT: This study explores methane utilization by the methanotrophic microorganism Methylococcus capsulatus (Bath) for biomass production, presenting a promising approach to mitigate methane emissions and foster the development sustainable biomaterials. Traditional screening methods for gas cultivations involve either serum flasks without online monitoring or costly, low-throughput fermenters. To address these limitations, the Respiration Activity MOnitoring System was augmented with methane sensors for real-time methane transfer rate (MTR) monitoring in shake flasks. Utilizing online monitoring of the MTR in shake flasks results in enhanced throughput and cost-effectiveness for cultivating M. capsulatus. Simultaneous monitoring of transfer rates for oxygen, methane, and carbon dioxide was conduc

SUBMITTER: Engel D 

PROVIDER: S-EPMC11632175 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature