Retinal organoid chip: engineering a physiomimetic oxygen gradient for optimizing long term culture of human retinal organoids.

ABSTRACT: An oxygen gradient across the retina plays a crucial role in its development and function. The inner retina resides in a hypoxic environment (2% O₂) adjacent to the vitreous cavity. Oxygenation levels rapidly increase towards the outer retina (18% O₂) at the choroid. In addition to retinal stratification, oxygen levels are critical for the health of retinal ganglion cells (RGCs), which relay visual information from the retina to the brain. Human stem cell derived retinal organoids are being engineered to mimic the structure and function of human retina for applications such as disease modeling, development of therapeutics, and cell replacement therapies. However, rapid degeneration of the retinal ganglion cell layers are a common limitation of human retinal organoid p

SUBMITTER: Drabbe E 

PROVIDER: S-EPMC11632457 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature