Click-ready iridium(iii) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling.

ABSTRACT: Herein, we report the synthesis, photophysical characterization and validation of iridium(iii)-polypyridine complexes functionalized for click chemistry and bioorthogonal chemistry, as well as their versatile applications as probes in bioimaging studies exploiting metabolic labeling. The designed dyes are conjugated to chemical reporters in a specific manner within cells by CuAAC ligation and display attractive photophysical properties in the UV-visible range. They are indeed highly photostable and emit in the far-red to near-IR region with long lifetimes and large Stokes shifts. We demonstrate that they can be efficiently used to monitor nascent intracellular sialylated glycoconjugates in bioorthogonal MOE studies with a varied panel of optical and non-optical techniques, namely conventio

SUBMITTER: Rigolot V 

PROVIDER: S-EPMC11632520 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature