Characterization of Photo-Crosslinked Methacrylated Type I Collagen as a Platform to Investigate the Lymphatic Endothelial Cell Response.

ABSTRACT: Despite chronic fibrosis occurring in many pathological conditions, few in vitro studies examine how fibrosis impacts lymphatic endothelial cell (LEC) behavior. This study examined stiffening profiles of PhotoCol^®-commercially available methacrylated type I collagen-photo-crosslinked with the photoinitiators: Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), Irgacure 2959 (IRG), and Ruthenium/Sodium Persulfate (Ru/SPS) prior to evaluating PhotoCol^® permeability and LEC response to PhotoCol^® at stiffnesses representing normal and fibrotic tissues. Ru/SPS produced the highest stiffness (~6 kilopascal (kPa)) for photo-crosslinked PhotoCol^®, but stiffness did not change with burst light exposures (30 and 90 s). The collagen fibril area fraction

SUBMITTER: Ruliffson BNK 

PROVIDER: S-EPMC11632916 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature