Deficiency of the histone H3K36 methyltransferase SETD2 inhibits the proliferation and migration of hepatocellular carcinoma cells.

ABSTRACT: Background: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. SETD2, the only known methyltransferase catalyzes the trimethylation of histone H3 lysine 36 (H3K36), has been reported to be associated with several cancers. However, the function of SETD2 in HCC is unclear. This work aimed to investigate the function and mechanism of SETD2 in HCC through bioinformatics analysis and cell experiments. Methods: SETD2 expression and its relationship with prognosis were evaluated in The Cancer Genome Atlas (TCGA)-LIHC cohort, and the effects of SETD2 silencing and overexpression on HCC cell lines were assesed via CCK-8, colony formation and wound healing assays. RNA-seq analysis, western blotting and chromatin immunoprecipitation (ChIP) ass

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11632984 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature