S100A4 targets PPP1CA/IL-17 to inhibit the senescence of sheep endometrial epithelial cells.

ABSTRACT:

Background

Gonadotropin-releasing hormone (GnRH) is commonly used in animal reproduction and production, but it was previously reported that GnRH decreases the embryo implantation rate during artificial insemination or embryo transfer in sheep. In addition to the finding that GnRH can target S100A4 to inhibit endometrial epithelial cells proliferation, it was also found that endometrial cells were in poor condition and experienced cell death in S100A4 knockout mice, but the mechanism is unclear.

Methods

The protein PPP1CA, which interacts with S100A4, was detected by immunoprecipitation-mass spectrometry of overexpression and knockdown of S100A4 and PPP1CA. The effect of S100A4 and PPP1CA on cell senescence was detected by Galactosidase staining. To further reveal the mechan

SUBMITTER: Jiao X 

PROVIDER: S-EPMC11633043 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature