Oligomerization-mediated phase separation in the nucleoid-associated sensory protein H-NS is controlled by ambient cues.

ABSTRACT: H-NS, a nucleoid-associated protein (NAP) from enterobacteria, regulates gene expression by dynamically transducing environmental cues to conformational assembly and DNA binding. In this work, we show that H-NS from Escherichia coli, which can assemble into octameric and tetrameric oligomerization states, forms spontaneous micron-sized liquid-like condensates with DNA at sub-physiological concentrations in vitro. The heterotypic condensates are metastable at 298 K, partially solubilizing with time, while still retaining their liquid-like properties. The condensates display UCST-like phase behavior solubilizing at higher temperatures, but with a large decrease in droplet-assembly propensities at 310 K and at higher ionic strength. Condensate formation can be tuned in a cyclic manner between

SUBMITTER: Lukose B 

PROVIDER: S-EPMC11633056 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature