A novel multiplex and glycoprotein-based immunochromatographic serologic IgM test for the rapid diagnosis of <i>Escherichia coli</i> O157 and O145 causing bloody diarrhea and hemolytic uremic syndrome.

ABSTRACT: Shiga toxin-producing Escherichia coli (STEC) are the main etiological agents of hemolytic uremic syndrome (HUS). Good clinical management of STEC infections and HUS depends on early, rapid, and accurate diagnosis. Here, we have developed and evaluated the first multiplex and glycoprotein-based immunochromatographic test for the detection of IgM antibodies against the O-polysaccharide of the lipopolysaccharide of E. coli O157 and O145 in human serum samples. A retrospective study was carried out resulting in a diagnostic sensitivity of the E. coli O157/O145 LFIA (lateral flow immunoassay) of 97.1% and 98.9% for O157 and O145, respectively, and 97.9% for both serogroups. The diagnostic specificity was 98.7% for O157 and O145, and the overall specificity 97.4%. In sample

SUBMITTER: Landivar SM 

PROVIDER: S-EPMC11633091 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature