Substrate identification of putative NCS1 and NCS2 nucleobase transporters in <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen that can salvage nucleobases from the environment to conserve nutrients that would otherwise be spent on de novo nucleotide biosynthesis. However, little is known regarding the substrate specificity of the 13 putative nucleobase transporters in P. aeruginosa. Here, using a combination of genetic and chemical approaches, we report substrate identifications for 10 putative nucleobase transporters in P. aeruginosa. Specifically, we individually expressed each transporter in a genetic background lacking all 13 putative nucleobase transporters and quantified growth on a panel of 10 nucleobases as sole nitrogen sources. We confirmed these expression-based substrate identifications using targeted genetic knockouts. In

SUBMITTER: Kennelly C 

PROVIDER: S-EPMC11633122 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature