Torque-speed relationship of the flagellar motor with dual-stator systems in <i>Pseudomonas aeruginosa</i>.

ABSTRACT: The single polar flagellar motor in Pseudomonas aeruginosa is equipped with two stator systems, MotAB and MotCD, both driven by H⁺ ions. The torque-speed relationship for flagellar motors with two stator systems has not been explored previously. In this study, we developed a method that utilizes optical trapping and fluorescence labeling to measure the torque-speed relationships for the wild-type P. aeruginosa motor with dual stators and mutant strains with a single stator system, revealing surprising differences in them. Moreover, we found that the MotAB stators exhibit slip-bond behavior in load dependence, contrasting with the catch-bond behavior of the MotCD stators and Escherichia coli stators. Further examination of the solvent isotope and pH effects o

SUBMITTER: Wu H 

PROVIDER: S-EPMC11633141 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature