A simplified pyrazinamidase test for <i>Mycobacterium tuberculosis</i> pyrazinamide antimicrobial susceptibility testing.

ABSTRACT: Pyrazinamide (PZA) is an important first-line drug for tuberculosis (TB) treatment by eradicating the persisting Mycobacterium tuberculosis complex (MTBC). Due to cost and technical challenges, end TB strategies are hampered by the lack of a simple and reliable culture-based PZA antimicrobial susceptibility testing (AST) for routine use. We initially developed a simplified chromogenic pyrazinamidase (PZase) test in the TB reference laboratory using a training set MTBC isolates with various drug-resistant profiles, and validated its performance using consecutive BACTEC MGIT 960 (MGIT)-culture-positive culture in 10 clinical laboratories. The pncA gene Sanger sequencing results were used as the reference, and compared to the MGIT-PZA AST. Differential diagnosis of Mycobacter

SUBMITTER: Chan H-H 

PROVIDER: S-EPMC11633146 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature