Unprecedented N₂O production by nitrate-ammonifying <i>Geobacteraceae</i> with distinctive N₂O isotopocule signatures.

ABSTRACT: Dissimilatory nitrate reduction to ammonium (DNRA), driven by nitrate-ammonifying bacteria, is an increasingly appreciated nitrogen-cycling pathway in terrestrial ecosystems. This process reportedly generates nitrous oxide (N₂O), a strong greenhouse gas with ozone-depleting effects. However, it remains poorly understood how N₂O is produced by environmental nitrate-ammonifiers and how to identify DNRA-derived N₂O. In this study, we characterize two novel enzymatic pathways responsible for N₂O production in Geobacteraceae strains, which are predominant nitrate-ammonifying bacteria in paddy soils. The first pathway involves a membrane-bound nitrate reductase (Nar) and a hybrid cluster protein complex (Hcp-Hcr) that catalyzes the conversion of

SUBMITTER: Xu Z 

PROVIDER: S-EPMC11633192 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature