<i>Vibrio cholerae</i> CsrA controls ToxR levels by increasing the stability and translation of <i>toxR</i> mRNA.

ABSTRACT: Intestinal colonization and virulence factor production in response to environmental cues is mediated through several regulatory factors in Vibrio cholerae, including the highly conserved RNA-binding global regulatory protein CsrA. We have shown previously that CsrA increases synthesis of the virulence-associated transcription factor ToxR in response to specific amino acids (NRES) and is required for the virulence of V. cholerae in the infant mouse model of cholera. In this study, we mapped the 5' untranslated region (5' UTR) of toxR and showed that CsrA can bind directly to an RNA sequence encompassing the 5' UTR, indicating that the regulation of ToxR levels by CsrA is direct. Consistent with this observation, the 5' UTR of toxR contains multiple putative CsrA

SUBMITTER: Mey AR 

PROVIDER: S-EPMC11633198 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature