Three transporters, including the novel Gai1 permease, drive amino acid uptake in <i>Histoplasma</i> yeasts.

ABSTRACT: The dimorphic fungus Histoplasma capsulatum, which almost exclusively resides within host phagocytic cells during infection, must meet its nutritional needs by scavenging molecules from the phagosome environment. The requirement for gluconeogenesis, but not fatty acid catabolism, for intracellular growth, implicates amino acids as a likely intracellular nutrient source. Consequently, we investigated Histoplasma growth on amino acids. Growth assays demonstrated that Histoplasma yeasts readily utilize most amino acids as nitrogen sources but only efficiently catabolize glutamine, glutamate, aspartate, proline, isoleucine, and alanine as carbon sources. An amino acid permease-based conserved domain search identified 28 putative amino acid transporters within the Histop

SUBMITTER: Ray SC 

PROVIDER: S-EPMC11633205 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature