Investigation of the inhibitory properties of azo-dyes on chorismate synthase from Paracoccidioides brasiliensis.

ABSTRACT: The efficient inhibition of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) by the broad-spectrum herbicide glyphosate validates the shikimate pathway as a promising target for developing antimicrobial, fungicidal and herbicidal agents. The last enzyme of this pathway, chorismate synthase (CS), catalyses an unusual reaction, making it an attractive target for novel inhibitors. Therefore, we tested a series of azo-dyes for their inhibitory potential against CS from the pathogenic fungus Paracoccidioides brasiliensis (PbCS) and identified the azo-dye PH011669 that exhibits a dissociation (K_d) and 50% inhibitory constant (IC₅₀) of 1.1 ± 0.1 and 10 ± 1 µM, respectively. Molecular docking and MD simulations provided insight into the mode of inhibit

SUBMITTER: Fuchs K 

PROVIDER: S-EPMC11633415 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature