Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.

ABSTRACT: Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman imaging techniques of cryofixed specimens to overcome SNR limitations by enabling long exposure of specimens under highly stabilized low-temperature conditions. The observation of frozen specimens in a cryostat at a constant low temperature immediately after rapid freezing enabled the improvement of SNR and enhanced the spatial and spectral resolution. We also confirmed that the cryofixation can preserve physicochemical states of specimens by observing alkyne-labeled coenzyme Q in cytosol and hemeproteins in acute ischemic myocardium, which cannot be done b

SUBMITTER: Mizushima K 

PROVIDER: S-EPMC11633761 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature