A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node.

ABSTRACT: Multi-organ-on-chip systems (MOOCs) have the potential to mimic communication between organ systems and reveal mechanisms of health and disease. However, many existing MOOCs are challenging for non-experts to implement due to complex tubing, electronics, or pump mechanisms. In addition, few MOOCs have incorporated immune organs such as the lymph node (LN), limiting their applicability to model critical events such as vaccination. Here we developed a 3D-printed, user-friendly device and companion tubing-free impeller pump with the capacity to co-culture two or more tissue samples, including a LN, under a recirculating common media. Native tissue structure and immune function were incorporated by maintaining slices of murine LN tissue ex vivo in 3D-printed mesh supports for at least 2

SUBMITTER: Cook SR 

PROVIDER: S-EPMC11633827 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature