Single-cell RNA sequencing reveals the CRTAC1⁺ population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1⁺ macrophage.

ABSTRACT: Degenerative spinal stenosis is a chronic disease that affects the spinal ligaments and associated bones, resulting in back pain and disorders of the limbs among the elderly population. There are few preventive strategies for such ligament degeneration. We here aimed to establish a comprehensive transcriptomic atlas of ligament tissues to identify high-priority targets for pharmaceutical treatment of ligament degeneration. Here, single-cell RNA sequencing was performed on six degenerative ligaments and three traumatic ligaments to understand tissue heterogeneity. After stringent quality control, high-quality data were obtained from 32,014 cells. Distinct cell clusters comprising stromal and immune cells were identified in ligament tissues. Among them, we noted that collagen degradation ass

SUBMITTER: Tang Y 

PROVIDER: S-EPMC11634701 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature