General anesthesia induces acute cell-free DNA methylation changes in peripheral blood.

ABSTRACT:

Background

Short-term and long-term adverse events could occur after general anesthesia (GA) and the specific mechanism driving these effects has not yet been well-characterized. In this study, we aimed to evaluate the global effect of GA on DNA methylation in the cell-free DNA (cfDNA) of surgical lung-nodule patients.

Methods

This large retrospective cohort study enrolled 1,006 surgical lung nodule patients (529 pre-anesthesia, and 477 post-anesthesia). Methylation profiles of the cfDNA isolated from plasma were analyzed by targeted bisulfite sequencing using an enrichment panel covering 12,899 biologically informative methylation regions and 105,844 CpG sites.

Results

By comparing the pre-anesthesia to the post-anesthesia group, a total of 4,562 differentially methy

SUBMITTER: Liang W 

PROVIDER: S-EPMC11635211 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature