Pathological investigation, molecular characterization and first-time isolation of the predominant serotypes of fowl adenovirus (FAdV-D and E) from commercial poultry in Bangladesh.

ABSTRACT:

Background

Fowl adenovirus (FAdV) is a globally distributed virus that inflicts significant economic losses on the poultry industry. The study aimed at pathological investigation, molecular characterization, isolation, and pathogenicity determination of FAdV from commercial poultry.

Methods

A total of 86 liver samples were collected from 80 commercial chicken farms. All samples were examined for gross pathology; only liver samples were used for histopathology and virus detection by PCR. PCR-positive FAdV samples were used for isolation of virus in 10-day-old seronegative embryonated chicken eggs (ECEs) via chorioallantoic membrane (CAM). PCR confirmed virus isolates were sequenced for serotyping and phylogenetic analysis. Pathogenicity of the isolated FAdVs was assessed by o

SUBMITTER: Sadekuzzaman M 

PROVIDER: S-EPMC11635299 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature