Role of Rab10 in cocaine-induced behavioral effects is associated with GABAB receptor membrane expression in the nucleus accumbens.

ABSTRACT:

Aim

Previous studies have demonstrated that Ras-related GTP-binding protein Rab10 (Rab10) plays a role in psychostimulant-induced behavioral effects. In this study, we showed that Rab10 in the nucleus accumbens (NAc) of male animals affects the development of cocaine-induced behavioral effects, which are associated with the plasma membrane expression of the GABA_B heteroreceptor (GABA_BR).

Methods

We performed flow cytometry, immunoendocytosis, pHluorin activity analysis, electrophysiology analysis, and open-field testing to explore the role of Rab10 in modulating the membrane expression and function of GABA_BR and its regulatory effect on cocaine-induced behavioral effects.

Results

Transcriptomics analysis showed that Rab10 was el

SUBMITTER: Yu Z 

PROVIDER: S-EPMC11635607 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature