STAG2 promotes naive-primed transition via activating Lin28a transcription in mouse embryonic stem cells.

ABSTRACT: Mouse embryonic stem cells (mESCs) exist in two distinct pluripotent states: the naive and the primed. Mainly by inducing differentiation of mESCs in vitro, conducting RNA sequencing analyses, and specifying expression of the regulatory genes, we explored the regulatory mechanisms underlying the transition between the naive and primed states. We found that, under the defined differentiation-inducing conditions, the naive state of mESCs shifted to the primed state within 2 days of differentiation induction, during which the cell cycle- and differentiation-related proteins changes significantly. Specifically, we uncovered that the expression of STAG2, a subunit of the Cohesin complex, was upregulated. We further revealed that knockout of STAG2 resulted in upregulation of the naive gene sets

SUBMITTER: Chen B 

PROVIDER: S-EPMC11635655 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature