External Cavity Quantum Cascade Laser Vibrational Circular Dichroism Spectroscopy for Fast and Sensitive Analysis of Proteins at Low Concentrations.

ABSTRACT: Proteins are characterized by their complex levels of structures, which in turn define their function. Understanding and evaluating these structures is therefore crucial to illuminating biological processes. One of the possible analytical methods is vibrational circular dichroism (VCD), which expands the structural sensitivity of classical infrared (IR) absorbance spectroscopy by the chiral sensitivity of circular dichroism. While this technique is powerful, it is plagued by low signal intensities and long measurement times. Here we present an optical setup leveraging the high brilliance of a quantum cascade laser to measure proteins in D₂O at a path length of 204 μm. It was compared to classical Fourier-transform infrared spectroscopy (FT-IR) in terms of noise levels and in its

SUBMITTER: Hermann DR 

PROVIDER: S-EPMC11635754 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature