Analyzing Binding Specificity in a Microparticle-Based DNA Displacement Assay Using Multiharmonic QCM-D.

ABSTRACT: Employing particles as a label is a common approach for signal amplification in various surface-based biosensors. However, the size dependency of adhesive forces can increase the likelihood of nonspecific interactions between the particles and surface. Hence, using microscale particles in surface-based sensors requires both developing surface chemistries with enhanced antifouling properties and precise methods for evaluating these properties. Here we employ a quartz crystal microbalance with dissipation monitoring (QCM-D) to investigate the binding specificity of microparticles anchored multivalently to DNA-functionalized surfaces. We design a competitive particle displacement assay by implementing toehold-mediated strand displacement as an actuator in the microparticle-substrate interface

SUBMITTER: Moazzenzade T 

PROVIDER: S-EPMC11636201 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature